Total Sugar Test Strips 65-650 ppm Reflectoquant 50 PK *Requires RQflex 2 EMD# 16950-1

Purchase Total Sugar Test Strips 65-650 ppm Reflectoquant 50 PK *Requires RQflex 2 EMD# 16950-1

Total Sugar Test Strips 65-650 ppm Reflectoquant   50 PK *Requires RQflex 2 EMD# 16950-1 MSDS (Material Safety Data Sheet)

Reflectoquant® Total Sugar Test, Product Inserts/General Instructions

Reflectoquant® Total Sugar
Test, Catalog No. 16136/1

1. Method

D-Glucose and D-fructose are converted into D-glucose-6-phosphate. This is oxidized by NAD under the catalytic effect of glucose-6-phosphate dehydrogenase to gluconate-6-phosphate. In the presence of diaphorase the NADH formed in the process reduces a tetrazolium salt to a blue formazan, the concentration of which is determined reflectometrically.

2. Measuring Range and Number of Determinations

Measuring Range Number of Determinations
65-650mg/l total sugar 50

3. Applications

Sample Material:
Beverages, e.g., wine, must
Food after appropriate sample pretreatment.

4. Influence of Foreign Substances

The determination is not interfered with by the concentrations of foreign substances usually present in wine.

Strongly colored samples (e.g., red wine) must be decolored by activated charcoal prior to the determination (see applications).

5. Reagents and Auxiliaries

The analytical test strips are stable up to the date stated on the pack when stored in the closed tube at +2 to +8°C.

Package Contents:
Tube containing 50 analytical test strips
1 Bar code strip
1 bottle of reagent TS-1
1 graduated 1ml plastic syringe
1 graduated 10ml plastic syringe
1 test vessel with stopper

Other Reagents:
D(-)-Fructose, Cat. No. 005321
Reflectoquant® Dilution Set, Cat. No. 17990
Stopwatch

6. Preparation

In certain cases it is necessary to dilute the sample to be analyzed (e.g., 1:10, i.e., 1ml of sample + 9ml of water) prior to the determination (see section 7).

Total Sugar Content
g/l
Dilution Dilution Factor
0.65 - 6.5
6.5 - 65
65 - 650
1 : 10
1 : 100
1 : 1000
10
100
1000

The Reflectoquant® Dilution Set is appropriate for this dilution.

7. Procedure

Observe the instructions for the reflectometer. The following applies to the Total Sugar Test:

Measurement Procedure A.
Stored reaction time: 600 sec.

Distilled water
(23+3°C)
10ml Inject into test vessel using syringe
Reagent TS-1 5 drops Add
Pretreated Sample
(23+3°C)
1ml Add with syringe and swirl
Press the START button of the reflectometer and - this is imperative - at the same time immerse both reaction zones of the analytical test strip in the measurement sample for approximately 2 seconds.

Allow excess liquid to run off via the long edge of the strip onto an absorbent paper towel.

Immediately insert the test strip all the way into the strip adapter with the reaction zones facing the display.

Read off the result from the display in °d.
The result is stored automatically.

Notes on Measurement:

  • Concerning the result of the analysis, the dilution (see section 6) must be taken into account:
    Result of analysis = measurement value x dilution factor
  • Serial Measurements: After the first measurement, it is possible to run further measurements by pressing the START button. In this case, however, a stopwatch is required since the countdown function of the reflectometer is available only once per series.

8. Method Control

To check analytical test strips, equipment, and procedure:
Dissolve 400mg of D(-)-fructose in distilled water, make up to 1000ml with distilled water, and mix.
Total sugar content: 400mg/l
Analyze this standard solution as described in section 7.

9. Notes

  • Reclose the reagent bottle and the tube containing the test strips immediately after use.
  • At the end of each workday, cleanse the strip adapter thoroughly with water or ethanol.

Purchase Total Sugar Test Strips 65-650 ppm Reflectoquant 50 PK *Requires RQflex 2 EMD# 16950-1

  • Gallade Part#: 161361
  • Mfr Part#: 16136-1
  • Brand: EMD Chemicals EM Science
  • Container: 1 PK of 50 Strips

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