Sulfite Test Strips 10-400 ppm EM Quant (100 Pack) 10013-1 EMD

Purchase Sulfite Test Strips 10-400 ppm EM Quant (100 Pack) 10013-1 EMD

Sulfite Test Strips 10-400 ppm EM Quant  (100 Pack) 10013-1 EMD MSDS (Material Safety Data Sheet)

10013 EM Quant® Sulfite Test

EM Quant® Sulfite Test, Catalog No. 10013

Test strips and reagent for the detection and semi-quantitative determination of sulfite ions

General

The Sulfite Test has useful application in food analysis, particularly in the testing of wine and fruit juices, sulfurated foods (e.g. fresh and dried fruit), fish, shellfish, crustaceans and minced meat products. Sulfur is often added to these foods as a preservative and antioxidant to improve their keeping properties. The substances in question are classified as E220 to 227 and are subject to statutory limits. Excessive concentrations of sulfite can provoke allergic reactions and discomfort in sensitive persons.

The test is also ideal for monitoring the sulfite excess in boiler and boiler feed waters, in which sulfite is used to bind (reduce) traces of oxygen.

Applications in the photographic industry include the testing of fixing baths, stop baths and developers containing potassium disulfite.

In waste water and in sewage treatment plants sulfite is harmful precisely because of its good bactericidal properties. It must not be discharged under any circumstances.

Principle of the Method

The reaction zone on the test strip is impregnated with sodium penta-cyanonitrosylferrate(II), potassium hexacyanoferrate(II) and zinc sulfate. It turns pink to brick red, depending on the concentration of sulfite ions present. The chemistry of this reaction is not fully understood. Surprisingly, the lowest concentration which can be reliably detected (10mg/l sulfite for the test strip) is one-sixth of the concentration that can be detected when the reaction occurs in a test tube.

Graduation

0 - 10 - 40 - 80 - 180 - 400mg/l (ppm) SO32–.

Directions

  1. Immerse a test strip in the sample solution for 1 second so that all four reaction zone is thoroughly moistened.
  2. Remove the test strip, gently shake off any surplus liquid, wait 30 seconds and compare the reaction zone with the color scale.

Note

The pH of the solution being tested should be between pH 6 and pH 12. At low pH values, less sulfite is indicated than is actually present. Solutions with a pH value below 6 must be adjusted to at least pH 7-10 with sodium acetate or sodium hydroxide solution.

Determination in Wine and Fruit Juices

Free Sulfite

  1. Immerse a test strip for 1 second so that the reaction zone is thoroughly moistened.
  2. Wait 30 seconds and compare the reaction zone with the color scale.
  3. Multiply the recorded value by 1.5.
Recorded Value Free Sulfite in mg/l
10 15
40 60
80 120

Total Sulfite

  1. Adjust the sample to pH 12 with sodium hydroxide solution.
  2. Immerse a test strip for 1 second so that the reaction zone is thoroughly moistened.
  3. Wait 30 seconds and compare the reaction zone with the color scale.

Measuring Sulfite in Air

The sulfite test strips are very sensitive to sulfur dioxide. To carry out this test, proceed as follows:

  1. Wet the reaction zone under running water, expose to the air for 5 minutes, then compare with the sulfite color scale.
  2. Wet the reaction zone of a fresh strip with the water used under 1 and compare immediately with the color scale (blank).
  3. Calculate the difference between 1 and 2 and divide by 2. The figure obtained is approximately equivalent to the sulfur dioxide content of the air (mg SO2/m3 air).

As little as 5mg SO2/m3 in air (~5 ppm) can be detected in this way.

NB: The maximum allowed concentration (MAC value) is 5mg SO2/m3 air (13ml SO2/m3).

Influence of Anions

The determination is not disturbed by less than

1000mg/l Br, BrO–3, Cl, ClO–3, ClO–4, CN, F, [Fe(CN)6]–4, I, IO–3, Mo7O6–24, NO–2, NO–3, OCN, PO3–4, S2O2–3, SO2–4, SeO2–3, SCN, VO–3, WO2–4, acetate, citrate, oxalate, succinate or tartrate.

Sulfite is oxidized to sulfate by various anions, e.g. CrO4–2, [Fe(CN)6]–3, IO–4, MnO–4 and S2O2–8.

Furthermore, it should be noted that sulfite is formed from S2O2–4 and S2O2–5 in aqueous solution and from S2O2–3 in acidic solution and is detected as such.

A yellow discoloration of the reaction zone is produced by more than 25mg/l N–3. More than 50mg/l S–2 causes the reaction zone to turn pink. This interference can be identified by the brownish-black coloration produced when a lead acetate paper moistened with water is held over the acidified solution.

Influence of Cations

The determination is not disturbed by less than

1000mg/l Al3+, As3+, Be2+, Bi3+, Ca2+, Cd2+, Ce3+, Co2+, Fe3+, Mg2+, Mn2+, NH4+, Rb+, Sb3+, Sr2+, Tl+, Zn2+, or Zr4+

If the pH value of the test solution is greater than 6, sulfite will be oxidized to sulfate in the presence of Ni2+ and atmospheric oxygen. Sulfite is also oxidized to sulfate by Au3+, Cr3+, Ce4+, Cu2+, Fe3+ and Hg+/2+.

A blue coloration of the reaction zone is caused by Fe3+, a brown coloration by Cu2+.

More than 100mg/l VO2+ produces a gray-brown discoloration of the reaction zone which cannot be eliminated.

Elimination of Interference

Elimination of interfering sulfide ions:

Mix 2 spatula-tips (approximately 0.4 g) of sodium acetate with 4ml of the sample solution. While shaking, add a cobalt(II) solution dropwise (mix a solution of 5 g cobalt(II) nitrate [Co(No3)2·6H2O] in 50ml water with a solution of 6 g trisodium citrate 2-hydrate in 50ml water) until a Co2+ concentration of 250-500mg/l is attained (check with EM Quant® Cobalt test #10002/1); sulfide can then no longer be detected with lead acetate paper. Filter or allow to settle and test the clear solution for sulfite as described above.

Standard (1000mg/l)

Dissolve 0.157g of sodium sulfite in 100ml of distilled water and stabilize with 0.04 g of Titriplex® III (EMD Chemicals product number 12029/30).

Package Size

10013 EM Quant® Pack of 100 test strips

Sulfite Test

Purchase Sulfite Test Strips 10-400 ppm EM Quant (100 Pack) 10013-1 EMD

  • Gallade Part#: 10013
  • Mfr Part#: 10013-1
  • Brand: EMD Chemicals EM Science
  • Container: 1 PK of 100 Strips

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