Reflectoquant® Peroxide Test, Catalog No. 16731/1
In the presence of peroxidase, hydrogen peroxide reacts with an organic redox indicator
to form a yellow-brown oxidation product, the concentration of which is determined reflectometrically.
2. Measuring Range and Number of Determinations
||Number of Determinations
|100 - 1000mg/l H2O2
1) for measurement of concentrations
>1000mg/l H2O2 see section 6
This test is suited for the determination of the hydrogen peroxide concentration in
4. Influence of Foreign Substances
This was checked - in the case of interfering
ions up to concentrations of 1,000mg/l - in solutions containing 400mg/l H2O2.
The concentrations of
foreign substances given in the table lie below the limit at which the
determination is interfered with:
|Concentrations of Foreign Substances in mg/l or °e|
||Free chlorine (hypochlorite)
||Bound chlorine (chloramine T)
5. Reagents and Auxiliaries
|The analytic test strips are stable at least
up to the date stated on the pack when stored in these conditions.
Tube containing 50 analytical test strips
Bar code strip
EM Quant® Peroxide Test, Cat. No. 10337, measuring range 100-1000mg/l H2O2
colorpHast® Universal Indicator strips pH 0-14, (non-bleeding), Cat. No. 9590
Titristar®Sodium hydroxide solution 1mol/l, Cat. No. SX0607H
Sulfuric acid 0.5mol/l, Cat. No. SX1243K
SUPRAPUR® Hydrogen Peroxide Solution, 30%, Cat. No. 7298
- Check whether the peroxide content is within the
measuring range. Use the EM Quant® Peroxide Test as a
guide. Samples containing more than 1000mg/l H2O2 must be diluted with
- The pH must be within the range 2-7. Check with Universal Indicator strips. If pH
is lower than 2, add sodium hydroxide solution dropwise; if greater than 7, add sulfuric acid
Observe the instructions for the RQflex
The following applies to the peroxide test:
Measurement procedure A
time: 60 sec.
- Remove 1 analytical test strip and immediately
reclose the tube.
- Press START button of the reflectometer and - this is imperative -
at the same time immerse both reaction zones of the test
strip in the measurement solution (15-25°C) for approximately 2 sec.
- Vigorously shake off excess liquid from the test strip
- As soon as the beeper sounds (5 sec. before
end of reaction time), insert the test strip all the way into the
strip adapter with the reaction zones facing the display.
- Read off the result from the display in mg/l H2O2. The
result is automatically stored.
- If the test strip is inserted into the
adapter after the reaction time has expired, renewed depression of
the START button may produce a false result
- At the end of each workday, cleanse the
strip adapter thoroughly with water or ethanol.
8. Method Control
To check analytical test strips, equipment and
Make up a 1ml Perhydrol® 30% H2O2
(= 333,000mg/l H2O2) to 1L with distilled water
and mix and analyze immediately (solution is not stable) as described
in section 7. The peroxide content determined should be 333mg/l.