EM Quant® Peroxide Test, Catalog No. 10337/1
In the presence of peroxidase, hydrogen peroxide reacts with an organic redox indicator to
form a yellow-brown oxidation product. The concentration of hydrogen peroxide is measured
semiquantitatively by visual comparison of the reaction zone
of the test strip with the fields of a color scale.
The reaction zone is at the extreme end of the test strip.
In addition, each test strip also possesses a warning zone. If this turns blue in color
(+), this means that the hydrogen peroxide concentration is greater than 2,000mg/l, although
the reaction zone may indicate a value within the measuring range! (See Note in Section 4.)
2. Measuring Range and Number of Determinations
|Measuring Range/Color Scale Graduation1)
||Number of Determinations
1) for measurement of concentrations
>1000mg/l H2O2 see section 6
This test is suited for the selective determination of the hydrogen peroxide
concentration in aqueous solutions.
4. Influence of Foreign Substances
This was checked - in the case of interfering ions up to concentrations
of 1000 mg/l - in solutions containing 400 and 0mg/l H2O2. The concentrations
of foreign substances given in the table lie below the limit at which
the determination is interfered with:
|Concentrations of Foreign Substances in mg/l or °e
|Free Chlorine (hypochlorite)
|Bound Chlorine (chloramine T)
The warning zone also turns blue in color in chlorine-containing solutions in which the
chlorine content is 10mg/l or greater!
5. Reagents and Auxiliaries
|The analytical test strips are stable at least up to the date stated on the pack when
stored in the closed tube at +2 to +8°C.
Tube containing 100 analytical test strips
colorpHast® pH Test Strips (non-bleeding), 0-14, Cat. No. 9590
Sodium hydroxide solution, 1mol/l, Cat. No. SX0706H
Sulfuric acid 0.5mol/l, Cat. No. SX1243H
- Samples containing more than 1000mg/l H2O2 must be diluted with distilled
- The pH must be within the range 2-7.
Check with colorpHast® strips.
If pH is lower than 2, add dilute sodium hydroxide solution dropwise; if greater than 7,
add dilute sulfuric acid dropwise.
- Remove 1 analytical test strip and immediately reclose the tube.
- Immerse both zones of the test strip in the measurement solution for
- Shake off excess liquid from the test strip.
- Wait 30 seconds, then compare the color of the reaction zone with the color
fields on the label. Determine with which field the color of the reaction zone coincides
most exactly and read off the corresponding concentration value. If an exact color match
cannot be achieved, estimate an intermediate value. The warning zone may not turn blue
in color during this time!