Nitrite Test Strips 1-80 ppm EM Quant (1 PK of 100)

Purchase Nitrite Test Strips 1-80 ppm EM Quant (1 PK of 100)

Nitrite Test Strips 1-80 ppm EM Quant  (1 PK of 100) MSDS (Material Safety Data Sheet)

10007 EM Quant® Nitrite Test

Purchase Nitrite Test Strips 1-80 ppm EM Quant (1 PK of 100)

  • Gallade Part#: 100071
  • Mfr Part#: 10007-1
  • Brand: EMD Chemicals EM Science
  • Container: 1 PK of 100 Strips

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  • EM Quant® Nitrite Test, Catalog No. 10007

    Test strips for the detection and semi-quantitative determination of nitrite ions

    General

    Nitrite indicates pollution. The EM Quant® Nitrite Test thus plays an important role in the analysis of drinking water, industrial water, effluent and aquarium water.

    Nitrite determination is an important aspect of food analysis and is applied in the testing of meat products, pickling salt, brine and deep frozen spinach. The test can also be used for the rapid analysis of skimmed milk, whey and powdered milk.

    Aquarium water should be tested for nitrite at regular intervals, as fish may be poisoned by concentrations exceeding 1mg/l (ppm), depending on species and duration of exposure. In bacteriology, the EM Quantâ Nitrite Test helps simplify the bacteriological nitrate reduction test.

    The test kit may also be used for measuring oxides of nitrogen in air.

    Principle of the Method

    Any nitrite contained in the test solution (the pH of which may be anywhere between 1 and 13) is converted to nitrous acid in the presence of an acid buffer. The nitrous acid diazolizes an aromatic amine and yields a diazonium salt, which couples with N-(1-naphthyl)ethylenediamine to produce a red-violet azo dye.

    Graduation

    0 - 1 - 5 - 10 - 20 - 40 - 80mg/l (PPM) NO2.

    Directions

    1. Remove a test strip and close the container immediately.
    2. Immerse the test strip in the sample solution for 1 second, so that the reaction zone is thoroughly moistened.
    3. Remove the test strip,gently shake off excess liquid, wait 15 seconds and compare the reaction zone with the color scale.

    Notes

    Strongly acidic solutions (pH <1) must be buffered to pH approximately 3-5 with sodium acetate; strongly alkaline solutions (pH >13) with tartaric acid. If the nitrite concentration is higher than 80mg/l, either dilute the sample or use 10022 EM Quant® Nitrite Test (measuring range 0.1-3g/l).

    10 mg NO2 is equivalent to 3 mg N.

    Determination in Meat Products

    Finely mince at least 10g of meat product several times in quick succession. Add 8ml of 1 N sodium acetate solution (13.6g sodium acetate dissolved in 100ml of distilled water) to 5g of the minced sample and homogenize for approximately 1 minute in a food blender or Ultra-Turrax stirrer. Filter through a soft fluted filter (pressinggently if necessary), collect in a short test tube and test for nitrite in the filtrate as directed on the pack.

    Determination in Sausage Meat

    Either use the method described above or follow the following simpler procedure:

    Use a mortar and pestle togrind 2g of finely chopped sausage for approximately 2 minutes with 5g of sea sand and 8ml of 1 N sodium acetate solution (see above). Then filter through a soft fluted filter (pressinggently if necessary), collect in a test tube or flask and test for nitrite as directed on the pack.

    The Nitrate Reductase Test for Meat and Meat Products

    The time required to reduce nitrate is a function of the quantity of reductases and thus also of the number of nitrate-reducing microorganisms (especially Enterobacteriaceae) present in slurries and homogenized specimens.

    To the slurried or homogenized meat add sufficient nitrate togive a final concentration of 0.06% then incubate at 30·C. Check for nitrate formation with a Nitrite Test strip at hourly intervals.

    Determination in Nitrite Pickling Salt

    Place 4g of nitrite pickling salt into a clean container with a capacity of approximately 2 liters. Add a small volume of water and swirl to dissolve the salt. Make up with water to a final volume of 1.5 liters and mix thoroughly. Briefly dip a nitrite strip in this solution, shake off any surplus water, wait 15 seconds and compare with color scale.

    If a value of 10mg/l is indicated, the pickling salt is satisfactory. It is unsatisfactory if the result is much higher or lower than this figure and must on no account be used if the nitrite content is too high.

    Influence of Anions

    The determination is not disturbed by less than

    1000mg/l Br, BrO3, Cl, ClO3, ClO4, CN, F, I, Mo7O246–, N3, NO3 OCN, PO43–, SO42–, SeO32–, WO42–, acetate, ascorbate, citrate, oxalate, succinate or tartrate.

    In the presence of more than 100mg/l [Fe(CN)6]4– , 25mg/l S2–, 100mg/l SCN, 500mg/l SO32–, 250mg/l S2O32– , 100mg/l S2O42– or 250mg/l S2O52– the nitrite concentration indicated is less than that actually present. This effect is even more pronounced at pH values of less than 6.

    Influence of Cations

    The determination is not disturbed by less than

    1000mg/l Ag+, Al3+, As3+, Ba2+, Be2+, Cd2+, Co2+, Cr3+, Cu2+, Fe2+, Hg+, Hg2+, K+, Li+, Mg2+, Mn2+, Na+, Ni2+, Pb2+, Rb+, Sb3+, Sn2+,Ti4+, Tl+, VO2+, Zn2+ or Zr4+.

    Special note: agray to brown discoloration of the reaction zone indicates the presence of oxidizing ions e.g. more than 10mg/l CrO42–, 25mg/l [Fe(CN)6]3–, 250mg/l IO3, 500mg/l IO4, 5mg/l MnO4, 100mg/l S2O82–, 10mg/l VO3, 25mg/l Au3+, 25mg/l Ce4+ or 100mg/l Fe3+. Nitrate is unstable in the presence of these ions.

    Standard

    19899 Nitrate standard solution, 500ml.

    Package Size

    10007 EM Quant® Pack of Nitrite Test 100 test strips

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