Nitrate Test Strips 10-500 ppm EM Quant (1 PK of 100)

Purchase Nitrate Test Strips 10-500 ppm EM Quant (1 PK of 100)

Nitrate Test Strips 10-500 ppm EM Quant (1 PK of 100) MSDS (Material Safety Data Sheet)

10050, 10020 EM Quant® Nitrate Test

Purchase Nitrate Test Strips 10-500 ppm EM Quant (1 PK of 100)

  • Gallade Part#: 100201
  • Mfr Part#: 10020-1
  • Brand: EMD Chemicals EM Science
  • Container: 1 PK of 100 Strips

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    • EM Quant® Nitrate Test, Catalog No. 10050, 10020

    Test strips and reagent for the detection and semi-quantitative determination of nitrate ions

    General

    The EM Quant® Nitrate Test provides a very simple, rapid, semi-quantitative means of determining nitrate in all aqueous media. There are many potential applications in the testing of drinking water, industrial water, aquarium water and waste water. High levels of nitrate in drinking water indicate that it is contaminated. A figure of 50mg/l (ppm) must not be exceeded in drinking water. In fresh water aquaria a level of 100mg/l (ppm) nitrate should not be exceeded.

    Nitrate-containing additives are frequently incorporated into dairy products and processed meats; as anti-swelling agents in cheese and as curing salt in meat products.

    Farm produce and fruit and vegetable juices often contain excessive amounts of nitrate because too much fertilizer has been applied to the crop. Apart from affecting quality and keeping properties, high concentrations of nitrate in foods can damage health.

    Hydroculture, agriculture and horticulture: In hydroculture, plants are fed with special fertilizers which provide a steady supply of nitrogen. The Nitrate Test can be used to check whether the supply is adequate. Also, the test can be used to measure nitrogen levels in the soil according to the mineralized nitrogen (NMIN) method.

    Principle of the Method

    A reducing agent reduces nitrate to nitrite. In the presence of an acid buffer, the nitrite is converted to nitrous acid, which diazotizes an aromatic amine. Coupling with N-(1-naphthyl)ethylenediamine produces a red-violet azo dye.

    Graduation

    0 - 10 - 25 - 50 - 100 - 250 - 500mg/l (ppm) NO3–

    Directions

    1. Remove a test strip and close the container immediately.
    2. Immerse the test strip in the sample solution for 1 second, so that the reaction zones are thoroughly moistened.
    3. Remove the test strip, gently shake off excess liquid, wait 1 minute and compare the reaction zones with the color scale.

    Notes

    There are two reaction zones on the test strip. The one furthest away from the tester's hand indicates both nitrate and nitrite, while the other indicates only nitrite. A pink to red-violet color appearing on the latter zone indicates the presence of interfering nitrite.

    The pH of the sample solution must be between 1 and 12.

    Strongly acidic solutions (pH <1) must be buffered with sodium acetate, strongly solutions (pH >12) with tartaric acid.

    Ten mg NO3 is equivalent to 2.3 mg N.

    The Nitrate Test is not a wholly satisfactory way of testing sea water, as the high salt concentration will produce false-lows.

    Use in Water Analysis

    The nitrate ion concentration in unpolluted natural waters does not generally exceed 3mg/l. Polluted water contains 50-150mg/l NO3 or more.

    Nitrate is the end product of nitrification. It results from the decay of plant and animal organisms containing nitrogen. High nitrate levels may also point to fecal contamination. Simultaneous tests for nitrite and ammonium will confirm or discount suspicions of this type of contamination. If the ammonium and nitrite concentrations are not found to be raised, the water is capable of mineralizing organic matter. The Nitrate Test is thus an ideal means of checking the effectiveness of sewage treatment processes and of testing aquarium filters.

    Use in the Testing of Foods

    The Nitrate Test plays an important role in the testing of foods such as milk and other dairy products, whey, brine, meat, potatoes, spinach and various other vegetables and vegetable juices. Meat products are cured with nitrate, while anti-selling agents containing nitrate are added to cheese.

    Testing potatoes for nitrate is simple. Make an incision in the potato, insert a test strip and squeeze the potato. Remove the strip after a few seconds, wait 1 minute and compare with t he color scale. Potatoes with levels of nitrate above 100mg/l have been over-dressed (i.e. over-fertilized). Such potatoes have inferior cooking and keeping properties. Over-dressing also reduces the starch content. Nitrate can be measured almost as easily in other types of produce, too. Chop a sample into several small pieces with a knife and squeeze in an onion or garlic press. Pipette off a defined volume of juice (e.g. 1-2ml) and dilute 10-fold or more with distilled water. Use a test strip to determine nitrate in the diluted juice. Multiply the result by the dilution factor. This gives the nitrate content in mg nitrate per kg of produce.

    Use in Agriculture and Hydroculture

    Use the Nitrate Test to measure the NMIN (mineralized nitrogen) requirement of the soil. Mix 10 g of a representative sample (collected with a core sampler from 30, 60 and 90cm below the surface) with 100ml of distilled or deionized water and stir vigorously to give a homogeneous mass without any lumps. Filter through a coffee filter or allow to settle, immerse a test strip in the filtrate or supernatant, and record the nitrate value shown on the scale. The nitrate value recorded is approximately equivalent to the value kg N/ha and 30cm soil later (ha = hectare). For example a value of 25mg/l NO3 read from the scale is equivalent to 25kg N/ha and 30cm soil layer. Farmers, market gardeners, vineyard owners, etc. can therefore apply fertilizer more discriminately. Apart from reducing their costs, they will also lessen the risk of ground water becoming polluted.

    Hydrocultures are fed with special fertilizers which ensure that the plants receive a steady supply of nitrogen. A Nitrate Test can be used to check whether the supply of nutrient is adequate.

    Periodic checks can be performed on crops, e.g. cereal crops, to monitor the current nitrogen supply situation. Equipment needed consists of a garlic press, a pipette and a small calibrated vessel, which is used to dilute the juice with distilled water (see also "Use in the testing of foods"). Fragments of the plant may also be brought into direct contact with the nitrate zone to measure the nitrate content at the contact surface.

    To determine nitrate in fodder crops, proceed as follows:

    1. Comminute the material with scissors or a knife to give pieces with a stalk length of approximately 1cm. Make sure that representative specimens are taken from all parts of the plant.
    2. Weigh out 50g of comminuted sample and reduce to pulp in an electric blender or mincer.
    3. Transfer pulp and juice to a beaker, rinse with 150ml of water and make up to exactly 500ml with warm water.
    4. Leave the pulp to stand for 10-15 minutes, stirring occasionally.
    5. Briefly dip a Nitrate Test strip in the supernatant or filtrate (filtered through a coffee filter) and assess the reaction zone color after 1 minute.
    6. Calculate the nitrate content of the crop:

    mg NO3/g crop =mg/l / 100

    Conversion to dry mass: mg NO3/g crop x 5 or 6 (5 for normal fodder crop with a dry substance content of approximately 20%; 6 for young crop with a dry substance content of approximately 16-17%).

    Nitrate determinations in fodder should be performed, in particular, during spring and autumn. This is because intensive dressing of green fodder followed by a drop in temperature may cause a several-fold increase in their nitrate content. Bulk storage of green fodder for protracted periods may have the same effect.

    Influence of Anions

    As little as 0.5mg/l of nitrite produces a false positive reaction.

    The determination is not disturbed by less than

    1000mg/l Br–, BrO3–, Cl–, ClO3–, ClO4–, CN–, F–, I–, Mo7O246–, N3–, OCN–, PO43–, SO42–, SeO42–, WO42–, acetate, ascorbate, citrate, oxalate, succinate or tartrate.

    In the presence of more than

    100mg/l [Fe(CN)6]4– , 25mg/l S2–, 100mg/l SCN–, 500mg/l SO32–, 250mg/l S2O32– 100mg/l S2O42– or 250mg/l S2O52– the nitrate concentration indicated is less than that actually present.

    Oxidizing anions turn the reaction zone light brown to orange brown.

    Interference is caused by more than

    20mg/l CrO42–, 100mg/l Fe(CN)63–, 500mg/l IO3–, 10mg/l MnO4, 100mg/l S2O82– or 25mg/l VO3–

    Influence of Cations

    The determination is not disturbed by less than

    1000mg/l Al3+, As3+, Ba2+, Be2+, Ca2+, Co2+, Cr3+, Cu2+, K+, Li+, Mg2+, Mn2+, Na+, Ni2+, Pb2+, Rb+, Sb3+, Sn2+,Ti+, Ti4+, Zn2+

    The color becomes weaker in the presence of more than

    500mg/l Fe2+, 250mg/l Fe3+, 50mg/l VO2+ or 500mg/l Zn4+

    More than 50mg/l Ag+, 50mg/l Hg+ or100mg/l Hg2+ interfere by turning the reaction zone gray.

    Elimination of Interference

    Elimination of interfering nitrite: Add 5 drops of 10% aqueous amidosulfuric acid solution to 5ml of sample solution (pH 10), shake several times and test for nitrate after 1 minute as described. This method allows as little as 10mg/l nitrate to be detected in the resence of 1000mg/l nitrite.

    Standard (1000mg/l)

    Nitrate standard solution, 500ml.

    Package Size

    10050 EM Quant® Pack of Nitrate Test 25 test strips

    10020 EM Quant® Pack of Nitrate Test 100 test strips
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