Lipase Test Strips 10-400) Reflectoquant 1 EA *Req. RQflex 2 #16950-1 HAZMAT fee incl

Purchase Lipase Test Strips 10-400) Reflectoquant 1 EA *Req. RQflex 2 #16950-1 HAZMAT fee incl

Lipase Test Strips 10-400) Reflectoquant   1 EA *Req. RQflex 2 #16950-1 HAZMAT fee incl MSDS (Material Safety Data Sheet)

Reflectoquant® Lipase Test, Cat No 5851

Purchase Lipase Test Strips 10-400) Reflectoquant 1 EA *Req. RQflex 2 #16950-1 HAZMAT fee incl

  • Gallade Part#: 5851-1
  • Mfr Part#: 5851-1
  • Brand: EMD Chemicals EM Science
  • Container: 1 PK of 50 Strips
  • Misc: HAZMAT UN1993 PGIII

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  • Reflectoquant® Lipase Test, Catalog No. 5851/1

    1. Method

    Lipases convert a specific substrate to form a blue dye, the concentration of which is determined reflectometrically.

    2. Measuring Range and Number of Determinations

    Measuring Range Number of Determinations
    10 - 400µg/l H1) 50
    4 - 160U/l

    1) relative to lipoprotein-lipase from Pseudomonas (400U/mg, acc. to the certificate of analysis for Merck KGaA, Darmstadt, Germany, Cat. No. 1.05389.0001) at 25°C.

    3. Applications

    Sample Material:

    Foods, e.g. milk and dairy products, cereals, and cereal products
    Biological samples (not for clinico-chemical diagnostics)

    4. Influence of Foreign Substances

    This was checked in solutions containing 200µg/l lipase. The concentrations of foreign substances given in the table lie below the limit at which the determination is interfered with:

    Concentrations of Foreign Substances in mg/l or %
    Ascorbic Acid 10 Benzoic Acid 0.25%
    Cr2O72- 100 Urea 5%
    Formaldehyde 200 NaCl 1%
    H2O2 100 Saccharose 5%
    I2 1 Sorbic Acid 1%

    Foreign activities in milk were tested using esterase from porcine liver. 100µg/l esterase yields a signal corresponding to approximately 13µg/l lipase.

    5. Reagents and Auxiliaries

    The analytical test strips are stable up to the date stated on the pack when stored in the closed tube at +2 to +8°C.
    Protect strips and reagents from light!

    Package Contents:

    Tube containing 50 analytical test strips
    1 Bar code strip "Lipase Milk"
    1 Bar code strip "Lipase"
    2 bottles of reagent Lip-1
    1 bottle of reagent Lip-2
    6 semi-microcuvettes

    Accessories: Block heater or water bath (25°C) or thermometer for the measurement of the ambient temperature
    Variable pipette for 1ml and 250µl volume
    Disposable test tubes for the dilution of the samples
    Stop-watch

    Note: The bar code strip "LIPASE-MILK" requires the RQflex 2 instrument. This bar code strip will not work in the older RQflex instruments.

    6. Preparation

    • Adjust the reagents Lip-1 and Lip-2 to room temperature (20-25°C) prior to measurement
    • For liquid milk samples use the bar-code strip "Lipase Milk" only; for all other samples use the bar-code strip "Lipase"
    • Extract solid sample materials by an appropriate method

    7. Procedure

    Observe the instructions for the RQflex Reflectometer.
    The following applies to the Lipase Test:

    Measurement procedure A
    Stored reaction time: 5 sec.

    • Fill reagent Lip-1 into the bottom part of the semi-microcuvette (0.8-1ml or approximately 40 drops, respectively) and adjust to 25°C (e.g. in a water bath)
    • Place 0.25ml of sample solution and 1ml of reagent Lip-2 in a disposable test tube and mix (dilution 1:5)
      In the case of raw milk, mix 0.1ml of sample solution and 1ml of reagent Lip-2
    • Remove 1 analytical test strip and immediately reclose the tube.
    • Immerse both reaction zones of the test strip in the test tube containing the diluted sample solution for approximately 2 seconds
    • Vigorously shake off excess liquid from the test strip
    • Within 10 seconds, place this test strip in the semi-microcuvette containing the pretreated and 25°C warm reagent solution and start stop-watch
    • After 15 minutes, remove the test strip immediately and vigorously shake off excess liquid
    • Switch on the RQflex reflectometer and press the START button once, then insert the test strip all the way into the strip adapter with the reaction zones facing the display
    • Immediately press the START button again. After the reaction time has elapsed, read off the result from the display in µg/l lipase.
      The result is stored automatically.

    8. Evaluation

    If the sample is analyzed at a temperature other than 25°C, it is necessary to correct the displayed result by a corresponding factor:

    Temperature
    (°C)
    Correction
    Factor
    Temperature
    (°C)
    Correction
    Factor
    15 2.2 23 1.2
    16 2.05 24 1.1
    17 1.9 25 1.0
    18 1.75 26 0.9
    19 1.6 27 0.85
    20 1.5 28 0.8
    21 1.4 29 0.75
    22 1.3 30 0.7

    The lipase values shown in the display are valid for UHT milk with 1.5% fat.
    For other types of milk, as well as for milk with a fat content other than 1.5%, it is necessary to correct the displayed result by a corresponding factor:

    Fat Content
    (%)
    Raw Milk UHT Milk Pasteurized
    Milk
    0 1.4 0.6 0.8
    0.5 1.5 0.7 0.9
    1.0 1.5 0.9 1.0
    1.5 1.5 1.0 1.2
    2.0 1.6 1.1 1.3
    2.5 1.6 1.3 1.4
    3.0 1.6 1.4 1.5
    3.5 1.6 1.5 1.6
    4.0 1.7 1.7 1.7
    4.5 1.7 1.8 1.8
    5.0 1.7 2.0 2.0
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