Reflectoquant® Iron Test Strips, Catalog No. 16982/1
1. Method
In acidic solution, Fe2+ ions react
with Ferrospectral® to form a red-violet complex, the
concentration of which is determined reflectometrically.
2. Measuring Range and Number of Determinations
| Measuring range1) |
Number of Determinations |
| 0.5 - 20.0mg/l Fe2+ |
50 |
1)for measurement of concentrations >
20.0mg/l Fe2+ see section 6
3. Applications
This test measures only Fe2+ ions.
Fe3+ ions must be reduced prior to the determination (see
section 7, "Notes").
Sample Material:
Surface water
Seawater
Industrial water
Wastewater
Mineral water
Food and food preserves (e.g. juices, wine,
preserve liquids)
4. Influence of Foreign Substances
This was checked - in the case of interfering
ions up to concentrations of 1,000mg/l - in solutions containing 10mg/l and 0mg/l Fe2+. The concentrations of foreign
substances given in the table lie below the limit at which the
determination is interfered with:
Concentrations of Foreign Substances in mg/l or %
| Ag+ |
100 |
| Al3+ |
1,000 |
| BO33- |
1,000 |
| Ca2+ |
1,000 |
| Cd2+ |
1,000 |
| Citrate |
1,000 |
| CN- |
10 |
| Cu2+ |
10 |
| Fe3+ |
1,000 |
| K+ |
1,000 |
| Mg2+ |
1,000 |
|
| Mn2+ |
1,000 |
| NH4+ |
1,000 |
| Ni2+ |
100 |
| NO2- |
100 |
| NO3- |
1,000 |
| Oxalate |
1,000 |
| Pb2+ |
1,000 |
| PO43- |
1,000 |
| S2O32- |
1,000 |
| Tartrate |
1,000 |
| Zn2+ |
1,000 |
|
| Anionic Surfactants1) |
1,000 |
| Cationic Surfactants2) |
1,000 |
| Nonionic Surfactants3) |
1,000 |
| NaCl |
20% |
| Na2SO4 |
20% |
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1)tested with Na-dodecyl sulfate
2)tested with N-cetylpyridinium
chloride
3)tested with polyvinylpyrrolidone
5. Reagents and Auxiliaries
The analytic test strips are stable at
least up to the date stated on the pack when stored in the closed
tube at +15 to +25°C.
Package Contents:
Tube containing 50 analytical test strips
Bar code strip
Other Reagents:
EM Quant® Iron Test, Cat. No.
10004, measuring range 3+500mg/l Fe2+
colorpHast® Universal indicator strips pH 0-14, Cat. No.
9590
Sodium acetate GR, Cat No. SX0255
Sulfuric acid 25% GR, Cat. No. SX1243M
L(+)Ascorbic acid GR, Cat. No. AX1775
Ammonium iron(II) sulfate hexahydrate GR, Cat. No. FX0245
6. Preparation
- Check whether the iron content is within the
measuring range
- Use the EM Quant® Iron Test
as a guide. Samples containing more than 20.0mg/l Fe2+
must be diluted with distilled water.
- The pH must be within the range 1-4.
Check with universal indicator strips. If pH is lower than 1, buffer
with sodium acetate; if greater than 4, add dilute sulfuric acid
dropwise.
7. Procedure
· Observe the instructions for the RQflex
Reflectometer.
The following applies to the Iron Test:
Measurement procedure A. Stored reaction time:
15 sec.
- Remove 1 analytical test strip and immediately
reclose the tube.
- Press START button of the reflectometer and
at the same time immerse both reaction zones of the test
strip in the pretreated sample solution (5-40°C) for
approx. 2 sec.
- Vigorously shake off excess liquid from the
test strip
- As soon as the beeper sounds (5 sec. before
end of reaction time), insert the test strip all the way into the
strip adapter with the reaction zones facing the display.
- Read off the result from the display inmg/l
Fe2+. The result is automatically stored.
Notes:
- At the end of each workday, cleanse the
strip adapter thoroughly with water or ethanol
- To determine the total of Fe2+
and Fe3+ ions, dissolve 1 spatula-tip of ascorbic acid
in approx. 10ml of sample solution and perform the measurement
after approx. 1 min.
9. Method Control
To check analytical test strips, equipment and
procedure:
Dissolve 0.7g ammonium iron(II) sulfate
hexahydrate in distilled water, make up to 100ml and mix. Fe2+
concentration 1,000mg/l. Dilute this standard solution to 10mg/l Fe2+
and analyze as described in section 7.
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MSDS
(Material Safety Data Sheet)
