Reflectoquant® Glucose Test Strips, Catalog No. 16720/1
Under the catalytic effect of glucose oxidase, glucose is converted to
gluconic acid lactone. The resultant hydrogen peroxide reacts with an
organic redox indicator in the presence of peroxidase to form a blue-green
dye, the concentration of which is determined reflectometrically.
2. Measuring Range and Number of Determinations
||Number of Determinations
|0-100 mg/l glucose
1) for measurement of concentrations
>100mg/l glucose see section 6
4. Influence of Foreign Substances
This was checked - in the case of interfering ions up to concentrations
of 1000 mg/l - in solutions containing 50mg/l glucose. The concentrations
of foreign substances given in the table lie below the limit at which
the determination is interfered with:
|Concentrations of Foreign Substances in mg/l
5. Reagents and Auxiliaries
|The analytical test strips are stable at least up to the date stated on the pack when
stored in the closed tube at +2 to +8°C.
Tube containing 50 analytical test strips
Bar code strip
colorpHast® pH-indicator strips non-bleeding, Universal Indicator pH 0-14, Cat. No. 9590
Titristar® Sodium hydroxide solution 1mol/l, Cat. No. SX0607.
Hydrochloric acid 1mol/l, Cat. No. HX0603D
D(+)-Glucose anhydrous, Cat. No. DX0145
Check whether the glucose content is within the
measuring range. Samples containing more than 100mg/l glucose must be diluted with distilled water.
The pH must be within the range 2-10. Check with Universal Indicator Strips. If pH is lower
than 2, add sodium hydroxide solution dropwise; if greater than 10, add hydrochloric acid
Observe the instructions for the RQflex
The following applies to the glucose test:
Measurement procedure A. Stored reaction
time: 60 sec.
- Remove 1 analytical test strip and immediately reclose the tube.
- Press START button of the reflectometer and
- this is imperative - at the same time place the test strip for 15 sec in the solution
to be tested (15-30°C) so that both reaction zones becomes completely wetted.
- Shake off excess liquid from the test strip
- As soon as the beeper sounds (5 sec. before
end of reaction time), insert the test strip all the way into the
strip adapter with the reaction zones facing the display.
- Read off the result from the display in mg/l glucose. The result
is automatically stored.
- If the test strip is inserted into the adapter after the reaction
time has expired, renewed depression of the START button may produce
a false result.
- At the end of each workday, cleanse the
strip adapter thoroughly with water or ethanol.
8. Method Control
To check analytical test strips, equipment and
Dissolve 0.1g of anhydrous D(+)-glucose in distilled water, make up to 100ml, and mix.
Glucose concentration 1000mg/l
Leave this standard solution to stand for 2 hours, then dilute to 50mg/l glucose and analyze
as described in section 7.