Reflectoquant® Calcium Test, Catalog No. 16993/1
1. Method
In the presence of hydrogen peroxide, Ca2+
ions react with glyoxal bis(2-hydroxyanil) to form a red complex, the
concentration of which is determined reflectometrically.
| Measuring range1) |
Number of Determinations |
| 2.5 - 45mg/l Ca2+ |
50 |
2. Measuring Range and Number of Determinations
1)for measurement of concentrations >
45.0 mg/l Ca2+ see section 6
3. Applications
Sample Material:
Groundwater and drinking water Industrial water
Wastewater Boiler feed water Beverages Food
4. Influence of Foreign Substances
This was checked - in the case of interfering
ions up to concentrations of 1,000mg/l - in solutions containing 20
and 0mg/l Ca2+. The concentrations of foreign substances
given in the table lie below the limit at which the determination is
interfered with:
Concentrations of Foreign Substances in mg/l
or %
| Acetate |
1,000 |
Fe3+ |
5 |
EDTA |
10 |
| Ag |
10 |
K+ |
1,000 |
Anionic Surfactants1) |
1,000 |
| Al3+ |
1,000 |
Mg2+ |
25 |
Cationic Surfactants2) |
1,000 |
| Ascorbate |
1,000 |
NH4+ |
1,000 |
Nonionic Surfactants3) |
1,000 |
| Br- |
1,000 |
Ni2+ |
5 |
H2O2 |
100 |
| Cd2+ |
1 |
NO2- |
1,000 |
|
|
| Cl- |
1,000 |
NO3 |
1,000 |
|
|
| CN- |
1,000 |
Oxalate |
1,000 |
|
|
| CO2+ |
5 |
Pb2+ |
1 |
|
|
| Cr3+ |
1 |
PO43- |
50 |
|
|
| CrO42- |
10 |
SO32- |
1,000 |
|
|
| Cu2+ |
5 |
Tartrate |
500 |
|
|
| Fe2- |
5 |
Zn2+ |
1 |
|
|
1) tested with Na-dodecyl sulfate
2) tested with N-cetylpyridinium
chloride
3) tested with polyvinyl pyrrolidone
5. Reagents and Auxiliaries
Please Note the Warnings on Labels!
The analytic test strips are stable at least up to the date stated on
the pack when stored in the closed tube at 15 to 25°·C.
Package Contents:
Tube containing 50 analytical test strips Bar code strip
1 bottle of reagent Ca–1 1 bottle of reagent Ca–2
1 test vessel 1 10-ml plastic syringe
Other Reagents:
EM Quant® Calcium Test, Cat. No.
10083, measuring range 10-100mg/l Ca2+
CertiPUR™ Calcium ICP Standard, 1000ppm, Cat. No. 1.70308.0100
6. Preparation
- Check whether the calcium content is within
the measuring range
Use the EM Quant® Calcium Test
as a guide. Samples containing more than 45 mg/l Ca2+ must
be diluted with distilled water.
7. Procedure
- Observe the instructions for the RQflex
Reflectometer.
The following applies to the Calcium Test:
Measurement procedure A. Stored reaction time:
90 sec.
Use the plastic syringe, transfer 6 ml of
pretreated sample solution (20-40ºC) to the test vessel.
- Remove 1 analytical test strip and immediately
reclose the tube.
- Press START button on reflectometer and at
the same time Immerse both reaction zones of the test strip
in the measurement solution for approx. 2 sec, then set
aside with the reaction zones facing upwards.
- Add 1 level blue microspoon (in the cap of
the Ca–1 bottle) of reagent Ca–1 to the sample solution
and dissolve by swirling.
- Add 10 drops of reagent Ca–2 and swirl.
- Press START button again of the
reflectometer and - this is imperative - at the same time
place the test strip in the measurement solution so that both
reaction zones become completely wetted.
- Exactly 10 secbefore the end of the
reaction time, remove the test strip from the solution and
vigorously shake off excess liquid. Then immediately insert
the test strip all the way into the strip adaptor with the reaction
zones facing the display.
- Read off the result from the display in mg/l
Ca2+. The result is automatically stored.
Note:
At the end of each workday, cleanse the strip
adapter thoroughly with water or ethanol
8. Method Control
To check analytical test strips, equipment and
procedure:
Dilute calcium standard solution to 10mg/l Ca2+
and analyze as described in section 7.
Purchase Calcium Test Strips 2.5-45 ppm Reflectoquant 1 PK *Req RQflex 2 #16950-1 HAZMAT fee incl
Gallade Part#: 16993
Mfr Part#: 16993-1
Brand: EMD Chemicals EM Science
Container: 1 PK of 50 Strips
Misc: HAZMAT UN3316 PGII CLASS 9
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