Reflectoquant® Ascorbic Acid Test Strips, Catalog No. 16981/1
1. Method
Ascorbic acid reduces yellow molybdophosphoric
acid to phosphomolybdenum blue, the concentration of which is
determined reflectometrically.
2. Measuring Range and Number of Determinations
| Measuring range1) |
Number of Determinations |
| 25-450mg/l ascorbic acid |
50 |
1) for measurement of concentrations
> 450mg/l ascorbic acid, see section 6
3. Applications
Sample Material:
Food and food preserves (e.g. fruit and
vegetable juices, beverages, beer, wine)
4. Influence of Foreign Substances
This was checked - in the case of interfering
ions up to concentrations of 1,000mg/l - in solutions containing 200mg
and 0mg/l ascorbic acid. The concentrations of foreign substances given in the table lie below the limit at which the determination is
interfered with:
Concentrations of Foreign Substances inmg/l
or %
| Citrate |
1,000 |
Oxalate |
1,000 |
| Fe2+ |
10 |
SO32- |
100 |
| Fe3+ |
10 |
Tartrate |
1,000 |
5. Reagents and Auxiliaries
| The analytical test strips are stable
at least up to the date stated on the pack when stored in the closed
tube at +15 to +25°C. |
Package Contents:
Tube containing 50 analytical test strips Bar code strip
Other Reagents:
EM Quant® Ascorbic Acid Test,
Cat. No. 10023, measuring range 50-2,000mg/l ascorbic acid
Ascorbic acid GR, Cat. No. AX1775
6. Preparation
- Check whether the ascorbic acid content is
within the measuring range
- Use the EM Quant® Ascorbic Acid Test as a guide. Samples
containing more than 450mg/l ascorbic acid must be diluted with distilled
water.
7. Procedure
- Observe the instructions for the RQflex Reflectometer.
The following applies to the Ascorbic Acid Test:
Measurement procedure A. Stored reaction time:
15 sec.
- Remove 1 analytical test strip and immediately
reclose the tube.
- Press START button of the Reflectometer and
at the same time immerse both reaction zones of the test
strip in the sample solution (5-30°C) for approx. 2 sec.
- Shake off excess liquid from the test strip
- As soon as the beeper sounds (5 sec. before
end of reaction time), insert the test strip all the way into the
strip adapter with the reaction zones facing the display.
- Read off the result from the display. The
result is automatically stored.
Note:
- If the test strip is inserted into the
adapter after the reaction time has expired, renewed depression of
the START button may result in a false reading of the ascorbic acid
concentration.
- At the end of each workday, the strip
adapter must be cleansed thoroughly with water or ethanol.
8. Method Control
To check analytical test strips, equipment and
procedure:
Dissolve 0.1g ascorbic acid in distilled water,
make up to 100ml, and mix. Ascorbic acid concentration 1,000mg/l.
Dilute this standard solution to 200mg/l
ascorbic acid and analyze as described in section 7.
Purchase Ascorbic Acid Test Strips 25-450 ppm Reflectoquant 50 PK *Requires RQflex 2 EMD# 16950-1
Gallade Part#: 169811
Mfr Part#: 16981-1
Brand: EMD Chemicals EM Science
Container: 1 PK of 50 Strips
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