Reflectoquant® Ammomium Test, Catalog No. 16899/1
1. Method
NH4+ ions react with a
chlorinating agent to form monochloramine. This in turn reacts with a
phenol compound to form a blue indophenol derivative, the
concentration of which is determined reflectometrically.
2. Measuring Range and Number of Determinations
| Measuring range1) |
Number of Determinations |
| 5.0-20.0mg/l NH4- |
50 |
1) measurement of concentrations > 20mg/l
NH4+ see section 6
3. Applications
Sample Material:
Wastewater Soils and fertilizers
4. Influence of Foreign Substances
This was checked - in the case of interfering ions up to concentrations
of 1,000 mg/l - in solutions containing 10mg/l NH4+.
The concentrations of foreign substances given in the table lie below
the limit at which the determination is interfered with:
Concentrations of foreign substances in mg/l or %
Al3+ 1,000
Ascorbate 100
BO33– 1000
Br– 1,000
Ca2+ 1,000
Citrate 1,000
Cl– 1,000
CN– 1
CO32– 1,000
Cr3+ 100
CrO42– 100
Cu2+ 100 |
Fe2+ 10
Fe3+
100
K+ 1,000
Mg2+ 1,000
Mn2+ 100
Ni2+ 1,000
NO2– 50
NO3– 1,000
Oxalate 1,000
PO43– 1,000
SO32– 100
Tartrate 1,000 |
EDTA 1,000
Anionic surfactants1) 1,000
Cationic surfactants2)1,000
Nonionic
surfactants3) 100
H2O2 10
Na2SO4 5% |
1) tested with Na-dodecyl sulfate
2) tested with N-cetylpyridinium
chloride
3) tested with polyvinylpyrrolidone
5. Reagents and Auxiliaries
| Please Note the Warnings on the packaging materials! |
| The analytical test strips are stable at least up to the date stated on the pack
when stored in the closed tube at +2 to +8°C. |
Package Contents:
Tube containing 50 analytical test strips Bar code strip 1 bottle of reagent NH4-1
1 bottle of reagent NH42- 1 test vessel
with stopper
Other Reagents:
EM Quant®
Ammonium Test, Cat. No. 10024, measuring range 10-400mg/l NH4+
colorpHast®
Universal Indicator Strips pH 0-14, Cat. No. 9590
Titristar®Sodium Hydroxide Solution 1mol/l, Cat. No. SX0607H
Titristar®Sulfuric
Acid Solution 0.5mol/l, Cat. No. SX1243H
Ammonium Standard Solution ready to use,
1000mg/l, NH4+, Cat. No. 19812
6. Preparation
- Check whether the ammonium content is within
the measuring range
Use the EM Quant® Ammonium Test as a guide. Samples
containing more than 20.0mg/l NH4+ must be diluted
with distilled water.
- The pH must be within the range 4-13. Check with pH indicator
strips. If pH is lower than 4, add sodium hydroxide solution dropwise;
if greater than 13, add sulfuric acid dropwise.
7. Procedure
Observe the instructions for the RQflex
Reflectometer.
The following applies to the Ammonium Test:
Measurement procedure A. Stored reaction time:
240 sec.
- Rinse the test vessel several times with the
pretreated sample solution (20-30°C). Then fill to the 5-ml
mark.
- Add 10 drops of reagent NH4 - 1
and swirl.
- Add 1 level blue microspoon (in the cap of
the NH4-2 bottle) of reagent NH4 -2 and swirl
to dissolve.
- Remove 1 analytical test strip and immediately
close the tube.
- Press START button of the reflectometer and - this is imperative
- at the same time place the test strip in the measurement solution
for 4 minutes.
- 10 seconds before end of reaction time, remove the test strip from the solution and vigorously
shake off excess liquid. Immediately insert the test strip all the way into the strip
adapter with the reaction zones facing the display.
- Read off the result from the display in mg/l
NH4+. The result is automatically stored.
Notes:
- If the test strip is inserted into the
adapter after the reaction time has expired, renewed depression of
the START button may result in a false reading of the ammonium
concentration.
- At the end of each workday, cleanse the
strip adapter thoroughly with water or ethanol.
8. Conversions
| Units Required |
Units Given |
Conversion Factor |
| mmol/m3NH4+ or N |
mg/l NH4+ |
55.4 |
| mg/l N |
mg/l NH4+ |
0.777 |
| mg/l NH4+ |
mmol/m3 NH4+ or N |
0.0180 |
| mg/l NH4+ |
mg/l N |
1.29 |
9. Method Control
To check analytical test strips, equipment, and
procedure:
Dilute ammonium standard solution to 10mg/l NH4+
and analyze as described in section 7.
Purchase Ammonium Test Strips 5.0-20.0 ppm Reflectoquant 1 PK *Req RQflex 2 #16950-1 HAZMAT fee incl
Gallade Part#: 16899-1
Mfr Part#: 16899-1
Brand: EMD Chemicals EM Science
Container: 1 PK of 50 Strips
Misc: HAZMAT UN3266 PGII CORROSIVE
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